The Interaction of Cycloserine with Glutamate Aspartate Transaminase as Measured by Fluorescence Spectroscopy

The interaction of cycloserine with glutamate aspartate transaminase as measured by fluorescence spectroscopy.

The reaction of cycloserine with pyridoxal5-phosphate has been investigated by fluorescence spectroscopy. The substantial change in fluorescence intensity at 450 rnp that follows the reaction of cycloserine with pyridoxal5-phosphate was used to monitor the release of the cofactor from the active site of the enzyme glutamate aspartate transaminase. A kinetic analysis of the fluorescence measurem...

Interaction between beta-fluoro-oxaloacetate and glutamate-aspartate transaminase of heart mitochondria.

Interaction between p-Fluoro-oxaloacetate and Glutamate- Aspartate Transaminase of Heart Mitochondria”

In a previous publication (1) we have described the synthesis and chemical properties of /3-fluoro-oxaloacetic acid. This acid was found to be a potent inhibitor of mitochondriil malic dehydrogenase (1,2). It is probable that fluoro-oxaloacetate inhibits other enzymes which react with oxaloacetate. We have performed further experiments in order to test this hypothesis. As a part of this study t...

Dffluoro-oxaloacetate with Aspartate Transaminase

Difluoro-oxaloacetate interacts with the aldimine form of aspartate transaminase to give a complex, the dissociation constant ofwhich has been determined spectrophotometrically and by 19F n.m.r. (nuclear magnetic resonance). The 19F n.m.r. line-width-pH and chemical-shift-pH profiles of difluoro-oxaloacetate in the presence of the aldimine form of the enzyme both show inflexion points in the pH...

Interaction of pyridoxal analogues with glutamate apodecarboxylase and aspartate apoaminotransferase.

The interaction of a variety of pyridoxal analogues with glutamate decarboxylase and aspartate aminotransferase has been studied. Pyridoxal phosphate N-oxide, N-methylpyridoxal phosphate, 3-0-methylpyridoxal phosphate, 4-de(formyl)-4-vinyl pyridoxal phosphate, and pyridoxal analogues which contain modified substituents in position 5 (CH2CHrOPOsHL, CH(CHB)OPOIH2, CHVPOBHL, and CHICH,COOH) are bo...